BB 607 - Proteomics: Principles and Techniques

Course content
  • An introduction to proteomics: Basics of protein structure and function, An overview of systems biology, Evolution from protein chemistry to proteomics
  • Abundance-based proteomics: Sample preparation and pre-fractionation steps, Gel-based proteomics - two-dimensional gel electrophoresis (2-DE), two-dimensional fluorescence difference in-gel electrophoresis (DIGE), Staining techniques
  • Central role of mass spectrometry: ionization sources, mass analyzers, different types of mass spectrometers; Quantitative proteomics - stable isotope labelling by amino acids in cell culture (SILAC), isotope-coded affinity tag (ICAT), isobaric tagging for relative and absolute quantitation (iTRAQ)
  • Functional proteomics: Recombinational cloning, Interactomics techniques to study protein-protein interactions, yeast two-hybrid, immunoprecipitation, protein microarrays, Nucleic Acid Programmable Protein Array (NAPPA)
  • Label-free nanotechnologies in proteomics, Surface Plasmon Resonance (SPR); Modificomics: understanding post-translational modifications; Structural proteomics; Bioinformatics in proteomics; Challenges and future prospects of proteomics research
  • Proteomics course will cover several basic concepts of various technologies but emphasis will be more on software's and hands-on sessions/ data analysis. 
  • Introduction to Proteomics: Tools for the New Biology, D.C. Liebler, Humana Press, 2002. 
  • Principles of Proteomics, R.M. Twyman, Bios Scientific Pub., 2004.
  • Proteomics for Biological Discovery, T.D. Veenstra, J.R. Yates III, John-Wiley & Sons, Hoboken, New Jersey, USA; 2006.
  • Protein Biochemistry and Proteomics (The Experimenter Series), R. Hubert, Academic Press, 2006. 
  • Proteomics in Practice: A Guide to Successful Experimental Design, R. Westermeier, T. Naven, H-R. Hopker, Wiley-VCH, 2008. 
  • Proteomics: A Cold Spring Harbor Laboratory Course Manual, A.J. Link and J. LaBaer, Cold Spring Harbor Laboratory Press, 2009. 
  • Selected papers from scientific journals.
Total credits : 6 credits – Lecture and Tutorial
Type : Department Elective
Duration : Full Semester